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egfp abi1  (Addgene inc)


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    Structured Review

    Addgene inc egfp abi1
    Egfp Abi1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+abi1/pEGFP+Abi1+(Plasmid+%2374905)/pmc12136925-398-21-28
    Average 93 stars, based on 7 article reviews
    egfp abi1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Generated:

    Article Title: Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics
    Article Snippet: pTriEx-CFP-PA-Rac1 was constructed by replacing the mVenus fragment in pTriEx-mVenus-PA-Rac1 (kindly gifted from Klaus Hahn; Addgene plasmid, #22007) with CFP. .. Lifeact-EGFP was generated by replacing the mCherry fragment in Lifeact-mCherry with EGFP. mCherry-Abi1 was constructed by replacing the Abi1 fragment from EGFP Abi1 (kindly gift from Giorgio Scita; Addgene plasmid, #74905) into the BglII/SacII site of pmCherry-C1 (TaKaRa). .. Before seeding the cells, 200 mesh gold holey carbon grids (R1.2/1.3; Quantifoil Micro Tools) were coated with 0.01% (w/v) poly- L -lysine (Nacalai Tesque, #28360-14), applied with 20 nm gold colloidal markers (Sigma-Aldrich, #741965).

    Construct:

    Article Title: Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics
    Article Snippet: pTriEx-CFP-PA-Rac1 was constructed by replacing the mVenus fragment in pTriEx-mVenus-PA-Rac1 (kindly gifted from Klaus Hahn; Addgene plasmid, #22007) with CFP. .. Lifeact-EGFP was generated by replacing the mCherry fragment in Lifeact-mCherry with EGFP. mCherry-Abi1 was constructed by replacing the Abi1 fragment from EGFP Abi1 (kindly gift from Giorgio Scita; Addgene plasmid, #74905) into the BglII/SacII site of pmCherry-C1 (TaKaRa). .. Before seeding the cells, 200 mesh gold holey carbon grids (R1.2/1.3; Quantifoil Micro Tools) were coated with 0.01% (w/v) poly- L -lysine (Nacalai Tesque, #28360-14), applied with 20 nm gold colloidal markers (Sigma-Aldrich, #741965).

    Plasmid Preparation:

    Article Title: Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics
    Article Snippet: pTriEx-CFP-PA-Rac1 was constructed by replacing the mVenus fragment in pTriEx-mVenus-PA-Rac1 (kindly gifted from Klaus Hahn; Addgene plasmid, #22007) with CFP. .. Lifeact-EGFP was generated by replacing the mCherry fragment in Lifeact-mCherry with EGFP. mCherry-Abi1 was constructed by replacing the Abi1 fragment from EGFP Abi1 (kindly gift from Giorgio Scita; Addgene plasmid, #74905) into the BglII/SacII site of pmCherry-C1 (TaKaRa). .. Before seeding the cells, 200 mesh gold holey carbon grids (R1.2/1.3; Quantifoil Micro Tools) were coated with 0.01% (w/v) poly- L -lysine (Nacalai Tesque, #28360-14), applied with 20 nm gold colloidal markers (Sigma-Aldrich, #741965).



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